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  3. 蛋白质组化

Recombinant Human Indian hedgehog protein

英文名：
Cas号：
检测信息查询
货号 规格 货期 库存 价格 会员价 订购
1308132717-10 μg 0 ￥3200 请登录
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1308132717-50 μg 0 ￥7800 请登录
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别 名
Cas号
M D L
分子式
分子量
产品参数
性状 Recombinant Human Indian hedgehog protein 能参与软骨细胞的分化、增值和成熟，特别是软骨内骨化的过程。
Synonyms
rHuIHH; HHG-2 ; 重组人印度刺猬蛋白
Species
Human

Source
E. coli

Accession
Q14623

Gene ID
3549

Molecular Weight
Approximately 20 kDa

AA Sequence
GPGRVVGSRR RPPRKLVPLA YKQFSPNVPE KTLGASGRYE GKIARSSERF KELTPNYNPD IIFKDEENTG ADRLMTQRCK DRLNSLAISV MNQWPGVKLR VTEGWDEDGH HSEESLHYEG RAVDITTSDR DRNKYGLLAR LAVEAGFDWV YYESKAHVHC SVKSEHSAAA KTGG

Biological Activity
The ED50 is <3 μg/mL as measured by its ability to induce alkaline phosphatase production by CCL-226 cells.

Appearance
Lyophilized powder.

Formulation
Lyophilized after extensive dialysis against PBS.

Endotoxin Level
<0.2 EU/μg, determined by LAL method.

Reconstitution
Reconstitute the lyophilized recombinant Human Indian hedgehog protein (rHuIHH) to 100 μg/mL using ddH2O or PBS.

Storage & Stability
Lyophilized recombinant Human Indian hedgehog protein (rHuIHH) is stored at -20°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping
Room temperature in continental US; may vary elsewhere.

Background
Indian hedgehog (Ihh), which encodes amember of the Hedgehog family of signaling factors, is initially expressed in chondrocytes of the early cartilaginous skeletal elements[1]. Indian Hedgehog is an antagonist of Wnt signaling in colonic epithelial cell differentiation[2]. Ihh is involved in chondrocyte differentiation, proliferation and maturation especially during endochondral ossification. It regulates its effects by feedback control of parathyroid hormone-related peptide (PTHrP) [3].
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