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Recombinant Human Interleukin-5
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| 性状 | Recombinant Human Interleukin-5 是一种选择性的人嗜酸性粒细胞功能激活剂。
Synonyms rHuIL-5; EDF; BCDFII; TRF Species Human Source E. coli Accession P05113 Gene ID 3567 Molecular Weight Approximately 26.5 kDa AA Sequence MIPTEIPTSA LVKETLALLS THRTLLIANE TLRIPVPVHK NHQLCTEEIF QGIGTLESQT VQGGTVERLF KNLSLIKKYI DGQKKKCGEE RRRVNQFLDY LQEFLGVMNT EWIIES Biological Activity The ED50 is <1.0 ng/mL as measured by TF-1 cells, corresponding to a specific activity of >1.0 × 106 units/mg. Appearance Lyophilized powder. Formulation Lyophilized after extensive dialysis against 25 mM Tris, pH 8.0. Endotoxin Level <0.2 EU/μg, determined by LAL method. Reconstitution Reconstitute the lyophilized recombinant Human Interleukin-5 (rHuIL-5) to 100 µg/mL using ddH2O. Storage & Stability Lyophilized recombinant Human Interleukin-5 (rHuIL-5) is stored at -20°C. After reconstitution, it is stable at 4°C for 2 weeks or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage. Shipping Room temperature in continental US; may vary elsewhere. Background Recombinant Human Interleukin-5 (rhIL-5) is a powerful and selective stimulator of human eosinophil function. IL-5 is thus the first hemopoietic factor whose elaboration in vivo can explain the selective eosinophilia and eosinophil activation seen in disease[1]. Recombinant human interleukin-5 (rhIL-5), in either liquid or semi- solid cultures, selectively induced eosinophil production from normal human bone marrow, with no activity on other cell lineages. Human interleukin-5 (IL-5) regulates the production of eosinophils in human bone marrowcultures: comparison and interaction with IL-1, IL-3, IL-6, and granulocyte-macrophage colony stimulating factor (GMCSF)[2]. |
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