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EpitomicsTandem™ IHC Staining and Detection Kits
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• Dual col o r IHC detection • Pair Epitomics RabMAbs with any mouse mAb • Optimized f o r use with Paraffin embedded tissues • Strong specific staining with clean background • Complete ready to use kits including detection reagents Intro Double staining is a technique used to evaluate the expression of two distinct antigens in the same tissue sample. The advent of Rabbit Monoclonal antibodies (RabMAbs) has opened up the possibility of perf o r ming reliable a n d clean dual IHC staining of f o r malin fixed, paraffin-embedded tissue samples. Until now, the maj o r ity of staining protocols involved biotinylated primary antibodies o r using a combination of monoclonal a n d polyclonal antibodies. These procedures generally provide inconsistent staining with high background. RabMAbs can be easily paired with mouse monoclonal antibodies in a straightf o r ward procedure that results in beautiful, clean dual labeled samples. T a n d em IHC Kits are offered with o r withour the primary rabbit monoclonal; Staining Kits include a rabbit monolconal primary antibody which have been specifically tested f o r use in dual col o r IHC detection. The Detection Kits contain the detection reagents only enabling researchers to pair any suitable Epitomics rabbit monoclonal antibody with any mouse monoclonal of their choice. Examples of dual col o r staining using Epitomics T a n d em IHC kits below. Each T a n d em IHC Kits is supplied with sufficient reagents to perfrom up to 50 stainings. |
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