| 质量标准： |
【产品介绍】ARF1 (small GTP-binding ADP-ribosylation
factor 1) is a member of the ARF family, which contains
five other ARF proteins (1). ARF1 functions in
various membrane trafficking events in the ER-Golgi
system and in the maintenance of organelle
structure. Inactive ARF1 (ARF1-GDP) localizes in
the cytoplasm, while the active form (ARF1-GTP) localizes
in the membrane (2-3). Active ARF1 is also localized to
the Golgi complex by regulating the assembly of
coat proteins onto membranes and mediating traffic in
the ER-Golgi. ARF1 activates phospholipase D
and phosphatidylinositol 4-phosphate 5-kinase, and
it recruits COPI to membranes (4-5).
Recommended Applications
WB, IHC, FC
Applications and Recommended Dilution Factors WB: 1:1,000 - 10,000
IHC: 1:250-500
FC: 1:80
Species Reactivity * Human, Mouse, Rat *Mouse cross reactivity tested by western blot and
IHC Rat cross reactivity tested by western blot only Products Data
A. Western blot analysis on 293 cell lysate using
anti-ARF1 RabMAb (cat. # 1635-1), dilution 1:2,000. B. Immunohistochemical analysis of paraffin-embedded
human breast carcinoma using anti-ARF1 RabMAb
(cat. # 1635-1). Specificity A synthetic peptide corresponding to residues in human
ARF1 was used as immunogen. This antibody is predicted
to detect splice isoforms 3, 4, 5 based on sequence
analysis. |
| 贮 存： |
Storage Condition and Buffer Store at -20 °C. Buffer: 50 mM Tris-Glycine (pH 7.4),
0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and
0.05% BSA. Stable for 12 months from date of receipt. |
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